From the microbiome to real-world impact: uncovering new CRISPR systems and applications

Our gene discovery, rooted in microbiome analysis, has led to novel Cas enzymes with unique properties. Designed for diagnostics and gene therapy, our enzyme enables multiplex detection through diverse probe recognition and offers stability, fast kinetics, and flexible targeting.

Continue expanding our CRISPR toolbox

Our Discovery Journey

  • Step 1- Metagenomic based Cas discovery

    Microbial DNAs were extracted, followed by metagenomic sequencing using next-generation sequencing (NGS) platforms. The resulting metagenomic data was mined to identify DNA sequences indicative of CRISPR systems

  • Step 2- Cas expression and purification

    Candidate Cas enzymes were cloned, expressed, and carefully purified for comprehensive downstream functional and structural characterization studies

  • Step 3- Characterisation of Cas enzymes

    The activity of each Cas enzyme in target recognition and signal generation was evaluated using CRISPR-based detection assays, and the most effective enzymes for gene editing and diagnostics applications